Associated with our newly established cryo-EM group at the CNPEM research laboratory (Campinas, Brazil), we have available a:
This position is available for 18 months (extendable to 36 months) and is aimed at developing advanced single-particle cryo-EM methodology. The position should be filled by the 1st of September 2017, or earlier if possible. (Other positions will become available at a later stage). We are looking for a creative and energetic colleague to study conformational variations as a means for better understanding the mechanisms of action of macromolecular complexes. As a national centre we study a variety of biological systems including small molecules (<100k), membrane proteins, large complexes, and viruses (Zika, Dengue). Candidates with backgrounds in the following fields are encouraged to apply: cryo-EM, X-ray crystallography, physics, biochemistry, biology, computer sciences, mathematics, and/or related fields. A sharp analytical mind and the ability to work in a group are essential. Programming experience in one or more (parallel) programming languages is important for the implementation of new ideas.
Our internationally operating research group has a long track record in methodology development (see: 2017 Wiley prize). Many of the standard procedures in single-particle cryo-EM stem originally from members of our group including: unsupervised classification; parallel MSA; Fourier Shell Correlation; projection matching; angular reconstitution; 4D cryo-EM, camera normalization; etc..
Data collection will take place on our own suites microscopes which currently includes a 200kV FEI Talos + Falcon III, and is expected to be extended with 300kV instrumentation with the latest generation of direct-electron camera Gatan K3/Falcon III. Access to other instrumentation is available through collaborations.
Marin van Heel